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Caspase 3 antibody

Anti-Caspase 3 antibody used in IHC (Paraffin sections) (IHC-P). GTX04908
Anti-Caspase 3 antibody used in Western Blot (WB). GTX04908
Anti-Caspase 3 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX04908
Anti-Caspase 3 antibody used in IHC (Paraffin sections) (IHC-P). GTX04908
Anti-Caspase 3 antibody used in Western Blot (WB). GTX04908
Anti-Caspase 3 antibody used in IHC (Paraffin sections) (IHC-P). GTX04908

Cat. No. GTX04908

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat, Bovine
Package
100 μl ($399)
See all Caspase 3 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:2000
ICC/IF 1:200
IHC-P 1:50-1:200
Not tested in other applications.

Calculated MW

32 kDa. ( Note )

Predict Reactivity

Rabbit, Sheep, Dog, Pig, Horse(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 150mM NaCl, 50% Glycerol

Preservative

0.02% Sodium Azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthesized peptide derived from human CASP3(Accession P42574), corresponding to amino acid residues I127-G177.

Purification


From serum

Purity

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

CASP3,CPP32,CPP32B,SCA1,caspase 3,Caspase 3

Cellular Localization

Cytoplasm

Background

The protein encoded by this gene is a cysteine-aspartic acid protease that plays a central role in the execution-phase of cell apoptosis. The encoded protein cleaves and inactivates poly(ADP-ribose) polymerase while it cleaves and activates sterol regulatory element binding proteins as well as caspases 6, 7, and 9. This protein itself is processed by caspases 8, 9, and 10. It is the predominant caspase involved in the cleavage of amyloid-beta 4A precursor protein, which is associated with neuronal death in Alzheimer's disease. [provided by RefSeq, Aug 2017]

Database

Research Area

DATA IMAGES

Anti-Caspase 3 antibody used in IHC (Paraffin sections) (IHC-P). GTX04908

GTX04908 IHC-P Image

IHC-P analysis of rat ovary tissue using GTX04908 Caspase 3 antibody.
Antigen retrieval : Heat mediated citrate buffer
Dilution : 1:100

Anti-Caspase 3 antibody used in Western Blot (WB). GTX04908

GTX04908 WB Image

WB analysis of various samples using GTX04908 Caspase 3 antibody.
Lane 1 : mouse brain tissue lysate
Lane 2 : rat spleen tissue lysate
Lane 3 : mouse liver tissue lysate
Lane 4 : mouse muscle tissue lysate

Anti-Caspase 3 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX04908

GTX04908 ICC/IF Image

ICC/IF analysis of PFA-fixed HeLa cells using GTX04908 Caspase 3 antibody.
Red : Primary antibody
Green : beta tubulin
Blue : DAPI
Dilution : 1:200
Permeabilization : 0.1% Triton X-100

Anti-Caspase 3 antibody used in IHC (Paraffin sections) (IHC-P). GTX04908

GTX04908 IHC-P Image

IHC-P analysis of rat lung tissue using GTX04908 Caspase 3 antibody.
Antigen retrieval : Heat mediated citrate buffer
Dilution : 1:100

Anti-Caspase 3 antibody used in Western Blot (WB). GTX04908

GTX04908 WB Image

WB analysis of A2780 whole cell lysate using GTX04908 Caspase 3 antibody. The lane on the left was treated with blocking peptide.

Anti-Caspase 3 antibody used in IHC (Paraffin sections) (IHC-P). GTX04908

GTX04908 IHC-P Image

IHC-P analysis of rat stomach tissue using GTX04908 Caspase 3 antibody.
Antigen retrieval : Heat mediated citrate buffer
Dilution : 1:100

REFERENCE

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REVIEW

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Package List Price ($)
$ 399