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Cathepsin K antibody, Internal (near N-term)

Anti-Cathepsin K antibody, Internal (near N-term) used in Western Blot (WB). GTX88744

Cat. No. GTX88744

Host

 Goat

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ELISA

Reactivity

 Human
Package
100 μg ($399)
See all Cathepsin K products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1-3μg/ml
ELISA Assay dependent
Not tested in other applications.

Calculated MW

37 kDa. ( Note )

Positive Control

The peptide used to generate this antibody is available for purchase (GTX88744-PEP).

Predict Reactivity

Mouse, Rat, Bovine, Dog, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

TBS, 0.5% BSA

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.50 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide with sequence C-KTHRKQYNNKVDE, from the internal region (near N Terminus) of the protein sequence according to NP_000387.1.

Purification

Purified by ammonium sulphate precipitation followed by antigen affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

cathepsin K , CTS02 , CTSO , CTSO1 , CTSO2 , PKND , PYCD

Cellular Localization

Lysosome

Background

The protein encoded by this gene is a lysosomal cysteine proteinase involved in bone remodeling and resorption. This protein, which is a member of the peptidase C1 protein family, is predominantly expressed in osteoclasts. However, the encoded protein is also expressed in a significant fraction of human breast cancers, where it could contribute to tumor invasiveness. Mutations in this gene are the cause of pycnodysostosis, an autosomal recessive disease characterized by osteosclerosis and short stature. [provided by RefSeq, Apr 2013]

Database

Research Area

DATA IMAGES

Anti-Cathepsin K antibody, Internal (near N-term) used in Western Blot (WB). GTX88744

GTX88744 WB Image

WB analysis of human lung lysate using GTX88744 Cathepsin K antibody, Internal (near N-term).
Dilution : 1μg/ml
Loading : 35μg protein in RIPA buffer

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399