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Cystatin B antibody [2F1]

Anti-Cystatin B antibody [2F1] used in Western Blot (WB). GTX53707

Cat. No. GTX53707

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 2F1

Isotype

 IgG2b

Application

 WB, ELISA

Reactivity

 Human
Package
100 μl ($399)
See all Cystatin B products

APPLICATION

Application Note

The antibody has been tested by ELISA and Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended dilution range for Western blot analysis is 1:1,000~ 2,000. Recommended starting dilution is 1:1,000.

Calculated MW

11 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.1% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant human Cystatin B (1-98aa) purified from E. coli

Purification

By protein-G affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

cystatin B , CPI-B , CST6 , EPM1 , EPM1A , PME , STFB , ULD

Cellular Localization

Cytoplasm , Nucleus

Background

The cystatin superfamily encompasses proteins that contain multiple cystatin-like sequences. Some of the members are active cysteine protease inhibitors, while others have lost or perhaps never acquired this inhibitory activity. There are three inhibitory families in the superfamily, including the type 1 cystatins (stefins), type 2 cystatins and kininogens. This gene encodes a stefin that functions as an intracellular thiol protease inhibitor. The protein is able to form a dimer stabilized by noncovalent forces, inhibiting papain and cathepsins l, h and b. The protein is thought to play a role in protecting against the proteases leaking from lysosomes. Evidence indicates that mutations in this gene are responsible for the primary defects in patients with progressive myoclonic epilepsy (EPM1). One type of mutation responsible for EPM1 is the expansion in the promoter region of this gene of a CCCCGCCCCGCG repeat from 2-3 copies to 30-78 copies. [provided by RefSeq, Jul 2016]

Database

Research Area

DATA IMAGES

Anti-Cystatin B antibody [2F1] used in Western Blot (WB). GTX53707

GTX53707 WB Image

WB analysis of A549 lysate (35ug) using Cystatin B antibody [2F1] at a dilution of 1:1,000.

REFERENCE

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399