APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
1-3μg/ml |
1-2μg/ml |
Note :
IHC-P
tonsil, stains scattered cells in the interfollicular area compatible with mast cells.
|
Not tested in other applications.
Calculated MW
Positive Control
The peptide used to generate this antibody is available for purchase (GTX89660-PEP).
Predict Reactivity
Mouse, Rat, Bovine, Dog, Pig(>80% identity)
PROPERTIES
Form
Liquid
Buffer
TBS, 0.5% BSA
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.50 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Peptide with sequence CLHRTETFPAYRSEH, from the C Terminus of the protein sequence according to NP_060901.2.
Purification
Purified by ammonium sulphate precipitation followed by antigen affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
docking protein 5 , C20orf180 , IRS-6 , IRS6
Background
The protein encoded by this gene is a member of the DOK family of membrane proteins, which are adapter proteins involved in signal transduction. The encoded protein interacts with phosphorylated receptor tyrosine kinases to mediate neurite outgrowth and activation of the MAP kinase pathway. Unlike other DOK family proteins, this protein does not interact with RASGAP. This protein is up-regulated in patients with systemic sclerosis and is associated with fibrosis induced by insulin-like growth factor binding protein 5. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Jun 2014]
Database
Research Area
DATA IMAGES
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GTX89660 IHC-P Image
IHC-P analysis of human tonsil using GTX89660 DOK5 antibody, C-term. Antigen retrieval : citrate buffer pH 6 Dilution : 1μg/ml
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GTX89660 WB Image
WB analysis of HEK293 overexpressing DOK5 (mock transfection in first lane) using GTX89660 DOK5 antibody, C-term.
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REFERENCE
There are currently no references for DOK5 antibody, C-term (GTX89660). Be the first to share your publications with this product.
REVIEW
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