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ERGIC3 antibody

Anti-ERGIC3 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX122511
Anti-ERGIC3 antibody used in Western Blot (WB). GTX122511
Anti-ERGIC3 antibody used in IHC (Paraffin sections) (IHC-P). GTX122511

Cat. No. GTX122511

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IHC-Fr

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all ERGIC3 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Fr Assay dependent
Not tested in other applications.

Calculated MW

43 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2

Predict Reactivity

Mouse, Rat, Zebrafish, Rabbit, Bovine, Xenopus tropicalis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.65 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human ERGIC3. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11168371

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

ERGIC and golgi 3 , C20orf47 , C2orf47 , CGI-54 , Erv46 , NY-BR-84 , PRO0989 , SDBCAG84 , dJ477O4.2

Cellular Localization

Endoplasmic reticulum-Golgi intermediate compartment membrane; Multi-pass membrane protein , Golgi apparatus , cis-Golgi network membrane; Multi-pass membrane protein , Endoplasmic reticulum membrane; Multi-pass membrane protein

Database

Research Area

DATA IMAGES

Anti-ERGIC3 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX122511

GTX122511 ICC/IF Image

ERGIC3 antibody detects ERGIC3 protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: ERGIC3 protein stained by ERGIC3 antibody (GTX122511) diluted at 1:1000.
Blue: Hoechst 33342 staining.
Anti-ERGIC3 antibody used in Western Blot (WB). GTX122511

GTX122511 WB Image

Sample (30 ug of whole cell lysate)
A: 293T
B: A431
C: HeLa
D: HepG2
10% SDS PAGE
GTX122511 diluted at 1:1000

Anti-ERGIC3 antibody used in IHC (Paraffin sections) (IHC-P). GTX122511

GTX122511 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human gastric cancer, using ERGIC3(GTX122511) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

Application Reference

Unger A et al. Int J Mol Sci 2023; 24 (7) Microscopic and Biochemical Hallmarks of BICD2-Associated Muscle Pathology toward the Evaluation of Novel Variants.
Application : IHC-Fr
Reactivity : Human
Submit a Reference

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169