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EphB4 antibody [N1N2], N-term

KO/KD
Anti-EphB4 antibody [N1N2], N-term used in Western Blot (WB). GTX108595
Anti-EphB4 antibody [N1N2], N-term used in Western Blot (WB). GTX108595

Cat. No. GTX108595

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all EphB4 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
Not tested in other applications.

Calculated MW

108 kDa. ( Note )

Positive Control

A431 , 293T

Predict Reactivity

Mouse, Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.63 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the Extracellular domain of human EphB4. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2036867

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

EPH receptor B4 , CMAVM2 , HFASD , HTK , LMPHM7 , MYK1 , TYRO11

Cellular Localization

Membrane; Single-pass type I membrane protein

Background

Ephrin receptors and their ligands, the ephrins, mediate numerous developmental processes, particularly in the nervous system. Based on their structures and sequence relationships, ephrins are divided into the ephrin-A (EFNA) class, which are anchored to the membrane by a glycosylphosphatidylinositol linkage, and the ephrin-B (EFNB) class, which are transmembrane proteins. The Eph family of receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. Ephrin receptors make up the largest subgroup of the receptor tyrosine kinase (RTK) family. The protein encoded by this gene binds to ephrin-B2 and plays an essential role in vascular development. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-EphB4 antibody [N1N2], N-term used in Western Blot (WB). GTX108595

GTX108595 WB Image

KO/KD

Wild-type (WT) and EphB4 knockout (KO) 293T cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with EphB4 antibody [N1N2], N-term (GTX108595) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-EphB4 antibody [N1N2], N-term used in Western Blot (WB). GTX108595

GTX108595 WB Image

Sample (30 ug of whole cell lysate)
A: A431 (GTX27909)
7.5% SDS PAGE
Eph receptor B4 antibody
GTX108595 diluted at 1:1000

REFERENCE

Application Reference

Bourgeois NM et al. Front Cell Infect Microbiol 2024; 14 : 1264525 Multiple receptor tyrosine kinases regulate dengue infection of hepatocytes.
Submit a Reference

REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169