Firefly Luciferase antibody [HL2164]

Cat. No. GTX638142

Host	Rabbit
Clonality	Monoclonal
Clone Name	HL2164
Isotype	IgG
Application	WB, ICC/IF
Reactivity	Firefly
Package	100 μl ($399), 25 μl ($169)

See all Firefly Luciferase products

Datasheet

View product citations for antibody GTX638142 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	Assay dependent
ICC/IF	Assay dependent

Not tested in other applications.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Firefly

Immunogen

Recombinant fragmemt of Firefly Luciferase

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Research Area

DATA IMAGES

GTX638142 ICC/IF Image

Firefly Luciferase antibody [HL2164] detects Firefly Luciferase protein by immunofluorescent analysis.
Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Firefly Luciferase stained by Firefly Luciferase antibody [HL2164] (GTX638142) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

GTX638142 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Firefly Luciferase antibody [HL2164] (GTX638142) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

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REVIEW

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Package

List Price ($)

100 μl

$ 399

25 μl

$ 169

Qty Custom Conjugations

Firefly Luciferase antibody [HL2164]

Cat. No. GTX638142

Host

Clonality

Clone Name

Isotype

Application

Reactivity