Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
1:1000-1:5000 |
1:500-1:2500 |
Assay dependent |
1:10000-1:50000 |
Not tested in other applications.
Product Note
Pre-adsorbed with Bovine, Horse, Human, Rabbit, Rat and Sheep serum proteins. May react with immunoglobulins from other species.
Form
Liquid
Buffer
20mM Potassium Phosphate, 150mM NaCl, 1% BSA
Preservative
0.01% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. Protect from light.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Mouse
Immunogen
Mouse IgG F(ab')2 fragment
Target Isotype
IgG F(ab')2
Purification
Purified by antigen-affinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorptions to remove any unwanted reactivities, pepsin digestion and chromatographic separation.
From serum
Conjugation
Fluorescein isothiocyanate (FITC)
Ratio : 2.8 molecules FITC per Goat IgG F(ab')2 molecule.
RRID
AB_378594
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.