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HIF3 alpha antibody

Orthogonal
Anti-HIF3 alpha antibody used in IHC (Paraffin sections) (IHC-P). GTX85702
Anti-HIF3 alpha antibody used in Western Blot (WB). GTX85702
Anti-HIF3 alpha antibody used in IHC (Paraffin sections) (IHC-P). GTX85702
Anti-HIF3 alpha antibody used in Western Blot (WB). GTX85702

Cat. No. GTX85702

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, ELISA

Reactivity

 Human, Mouse
Package
50 μg ($399)
See all HIF3 alpha products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:8000
IHC-P Assay dependent
ELISA 1:14000-1:80000
Not tested in other applications.

Calculated MW

73 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

20mM Potassium Phosphate, 150mM NaCl

Preservative

0.01% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

Synthetic peptide corresponding to an internal region near aa 575-600 of mouse Hif3 alpha (Hypoxia Inducible Factor).

Purification

Purified by antigen-affinity chromatography.
From serum

Conjugation

Unconjugated

RRID

AB_10725204

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

hypoxia inducible factor 3, alpha subunit , Ipas , MOP7 , NEPAS , bHLHe17

Cellular Localization

Nucleus,Cytoplasm,Nucleus speckle,Mitochondrion

Database

Research Area

DATA IMAGES

Anti-HIF3 alpha antibody used in IHC (Paraffin sections) (IHC-P). GTX85702

GTX85702 IHC-P Image

Human Placenta (formalin-fixed, paraffin-embedded) stained with HIF3A antibody (GTX85702) at 5 ug/ml followed by biotinylated goat anti-rabbit IgG secondary antibody LS-D1, alkaline phosphatase-streptavidin and chromogen.

Anti-HIF3 alpha antibody used in Western Blot (WB). GTX85702

GTX85702 WB Image

Anti-Hif Antibody - Western Blot. Western blot of Affinity Purified anti-Hif antibody shows detection of a band ~72 kD corresponding to mouse Hif3a (arrowhead). Approximately 10 ug of a CoCl2 treated 3T3 cell lysate (lane 1) and control 3T3 cell lysate (lane 2) were separated by 4-20% SDS-PAGE and transferred onto nitrocellulose. Treatment of exponentially growing 3T3 cells with 130 uM CoCl2 for 6 h at 37 degree C effectively mimics hypoxia. After blocking the membrane was probed overnight at 4C with the primary antibody diluted to 1:1600. The membrane was washed and reacted with a 1:10000 dilution of IRDye800 conjugated Gt-a-Rabbit IgG [H&L] MX ( for 45 min at room temperature. IRDye800 fluorescence image was captured using the Odyssey Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.

Anti-HIF3 alpha antibody used in IHC (Paraffin sections) (IHC-P). GTX85702

GTX85702 IHC-P Image

Human Lung, Respiratory Epithelium (formalin-fixed, paraffin-embedded) stained with HIF3A antibody (GTX85702) at 5 ug/ml followed by biotinylated goat anti-rabbit IgG secondary antibody LS-D1, alkaline phosphatase-streptavidin and chromogen.

Anti-HIF3 alpha antibody used in Western Blot (WB). GTX85702

GTX85702 WB Image

Orthogonal

WB analysis of CoCl₂-treated (lane 1) and untreated (lane 2) NIH-3T3 whole cell lysate using GTX85702 HIF3 alpha antibody.
Loading : 10 μg
Dilution : 1:1600

REFERENCE

Application Reference

Qu X et al. Anal Cell Pathol (Amst) 2022; 2022 : 3066456 XBP1 Regulates the Transcription of HIF-1a in BALB/c Mice with Chronic Rhinosinusitis without Polyps.
Application : WB
Reactivity : Mouse
Sun DG et al. Front Endocrinol (Lausanne) 2020; 11 : 169 The miRNA-29b Is Downregulated in Placenta During Gestational Diabetes Mellitus and May Alter Placenta Development by Regulating Trophoblast Migration and Invasion Through a HIF3A-Dependent Mechanism.
Application : IHC-P
Reactivity : Human
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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399