Application Note
For ELISA: Use at an assay dependent dilution. Reactivity against HIV1 >10-5. For WB: Use at a concentration of 0.1-1 μg/ml. This concentration will allow visualization of 100ng/lane of recombinant HIV1 p24. Optimal dilutions/concentrations should be determined by the researcher.
Product Note
This antiboy reacts with HIV1 P24 (core protein). There's no cross reactivity with HIV1 GP41 and HIV2 GP36.
Form
Liquid
Buffer
PBS
Preservative
No preservative
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human Immunodeficiency virus
Immunogen
Purified recombinant HIV1 p24 (core protein).
Purification
Protein G purified
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
Synonyms
Human immunodeficiency virus p24 , HIV p24
Cellular Localization
Nuclear
Background
HIV1 performs highly complex orchestrated tasks during the assembly, budding, maturation, and infection stages of the viral replication cycle. During viral assembly, the proteins form membrane associations and self-associations that ultimately result in budding of an immature virion from the infected cell. Gag precursors also function during viral assembly to selectively bind and package two plus strands of genomic RNA.
Research Area