PRODUCT
Summary
ITCH peptide is used for blocking the activity of ITCH antibody (catalog number GTX17213). It usually blocks the antibody activity completely in Western blot by incubating the peptide with equal volume of antibody for 30 min at 37ºC.
APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
Assay dependent |
Not tested in other applications.
PROPERTIES
Form
Liquid
Buffer
PBS, 0.1% BSA
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Aliquot and store at -20ºC or below. Avoid freeze-thaw cycles.
Concentration
200 μg/ml (Please refer to the vial label for the specific concentration.)
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
Itchy E3 Ubiquitin Protein Ligase , Admfd , Aif4 , Aip4 , Napp1 , Itch
Background
This gene encodes a member of the Nedd4 family of HECT domain E3 ubiquitin ligases. HECT domain E3 ubiquitin ligases transfer ubiquitin from E2 ubiquitin-conjugating enzymes to protein substrates, thus targeting specific proteins for lysosomal degradation. The encoded protein plays a role in multiple cellular processes including erythroid and lymphoid cell differentiation and the regulation of immune responses. Mutations in this gene are a cause of syndromic multisystem autoimmune disease. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Mar 2012]
Research Area
REFERENCE
There are currently no references for ITCH blocking peptide (GTX17213-PEP). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for ITCH blocking peptide (GTX17213-PEP). Be the first to share your experience with this product.