*Optimal dilutions/concentrations should be determined by the researcher.
Application
Recommended Dilution
WB
Assay dependent
ICC/IF
Assay dependent
IHC-P
Assay dependent
IHC-Fr
Assay dependent
FACS
1/50-1/100
Note :
FACS
Use 10μl of the suggested working dilution to label 106 cells in 100μl.
Not tested in other applications.
Product Note
This antibody recognizes the Ly-6B.2 antigen in 129J; AKR; C57BL/6; C57BL/10; C58; DBA/2; NZB; NZW; SJL; MFI; Swiss (PO) Strains whilst A2G; A/Sn; ASW; BALB/c; C3H/HEH: CBA.T6T6 are negative or demonstrate very weak reactivity (Hirsch and Gordon 1982).
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
0.09% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Mouse
Immunogen
Cultured bone marrow cells
Purification
Protein G purified From tissue culture supernatant
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
FACS analysis of mouse peripheral blood granulocytes using GTX39794 Ly-6B.2 antibody [7/4].
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GTX39794 IHC-Fr Image
IHC-Fr analysis of mouse lymph node tissue using GTX39794 Ly-6B.2 antibody [7/4].
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GTX39794 IHC-Fr Image
IHC-Fr analysis of mouse lymph node tissue using GTX39794 Ly-6B.2 antibody [7/4].
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GTX39794 IHC-Fr Image
IHC-Fr analysis of C57BL/6 mouse spleen tissue using GTX39794 Ly-6B.2 antibody [7/4].
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GTX39794 IHC-Fr Image
IHC-Fr analysis of mouse lymph node tissue using GTX397943 Ly-6B.2 antibody, green in A and Rat anti Mouse CD8 antibody, clone YTS105.18, red in B. C is the merged image with nuclei counterstained blue using DAPI.
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GTX39794 IHC-Fr Image
IHC-Fr analysis of mouse lymph node tissue using GTX39794 Ly-6B.2 antibody [7/4].
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GTX39794 IHC-Fr Image
IHC-Fr analysis of mouse lymph node tissue using GTX397943 Ly-6B.2 antibody, green in A and Rat anti Mouse CD8 antibody, clone YTS105.18, red in B. C is the merged image with nuclei counterstained blue using DAPI.