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Lysyl-tRNA synthetase antibody [8G12C1]

Anti-Lysyl-tRNA synthetase antibody [8G12C1] used in IHC (Paraffin sections) (IHC-P). GTX83186
Anti-Lysyl-tRNA synthetase antibody [8G12C1] used in Western Blot (WB). GTX83186
Anti-Lysyl-tRNA synthetase antibody [8G12C1] used in IHC (Paraffin sections) (IHC-P). GTX83186

Cat. No. GTX83186

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 8G12C1

Isotype

 IgG1

Application

 WB, IHC-P, ELISA

Reactivity

 Human
Package
100 μl ($399)
See all Lysyl-tRNA synthetase products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1/500 - 1/2000
IHC-P 1/200 - 1/1000
ELISA 1/10000
Not tested in other applications.

Calculated MW

68 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

Ascites

Preservative

0.03% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Purified recombinant fragment of Lysyl-tRNA synthetase(aa90-174) expressed in E. Coli.

Purification

Unpurified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

lysyl-tRNA synthetase , CMTRIB , DFNB89 , KARS1 , KARS2 , KRS

Cellular Localization

Cytoplasm,Nucleus,Cell membrane,Secreted

Background

Aminoacyl-tRNA synthetases are a class of enzymes that charge tRNAs with their cognate amino acids. Lysyl-tRNA synthetase is a homodimer localized to the cytoplasm which belongs to the class II family of tRNA synthetases. It has been shown to be a target of autoantibodies in the human autoimmune diseases, polymyositis or dermatomyositis. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-Lysyl-tRNA synthetase antibody [8G12C1] used in IHC (Paraffin sections) (IHC-P). GTX83186

GTX83186 IHC-P Image

IHC-P analysis of human Tonsil tissue using GTX83186 Lysyl-tRNA synthetase antibody [8G12C1].

Anti-Lysyl-tRNA synthetase antibody [8G12C1] used in Western Blot (WB). GTX83186

GTX83186 WB Image

WB analysis of truncated Lysyl-tRNA synthetase recombinant protein (1),truncated MBPLysyl-tRNA synthetase (aa90-174) and Lysyl-tRNA synthetase (aa1-188) transfected CHO-K1 cell lysate (3) using GTX83186 Lysyl-tRNA synthetase antibody [8G12C1].

Anti-Lysyl-tRNA synthetase antibody [8G12C1] used in IHC (Paraffin sections) (IHC-P). GTX83186

GTX83186 IHC-P Image

IHC-P analysis of human cervical carcinoma using GTX83186 Lysyl-tRNA synthetase antibody [8G12C1].

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399