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NEDD4 antibody

Orthogonal
Anti-NEDD4 antibody used in Western Blot (WB). GTX134444
Anti-NEDD4 antibody used in Western Blot (WB). GTX134444

Cat. No. GTX134444

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all NEDD4 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000
Not tested in other applications.

Calculated MW

102 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

2.17 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Rat

Immunogen

Recombinant protein encompassing a sequence within the center region of rat NEDD4. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2887283

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

neural precursor cell expressed, developmentally down-regulated 4, E3 ubiquitin protein ligase , Nedd4a

Cellular Localization

Cytoplasm,Cell membrane

Background

may regulate function of the amiloride-sensitive epithelial sodium channel ENaC [RGD, Feb 2006]

Database

Research Area

DATA IMAGES

Anti-NEDD4 antibody used in Western Blot (WB). GTX134444

GTX134444 WB Image

Various tissue extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with NEDD4 antibody (GTX134444) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
The observed M.W. is based on the publication: PMID: 26304770

Anti-NEDD4 antibody used in Western Blot (WB). GTX134444

GTX134444 WB Image

Orthogonal

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with NEDD4 antibody (GTX134444) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169