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NRF2 antibody [HL3183]

Recombinant antibody Orthogonal
Anti-NRF2 antibody [HL3183] used in Western Blot (WB). GTX640822
Anti-NRF2 antibody [HL3183] used in Western Blot (WB). GTX640822
Anti-NRF2 antibody [HL3183] used in Western Blot (WB). GTX640822

Cat. No. GTX640822

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 HL3183

Isotype

 IgG

Application

 WB

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all NRF2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
Not tested in other applications.

Calculated MW

68 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No Preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fragment of human NRF2.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

nuclear factor, erythroid 2 like 2 , HEBP1 , IMDDHH , NRF2

Cellular Localization

Cytoplasm, cytosol,Nucleus

Background

This gene encodes a transcription factor which is a member of a small family of basic leucine zipper (bZIP) proteins. The encoded transcription factor regulates genes which contain antioxidant response elements (ARE) in their promoters; many of these genes encode proteins involved in response to injury and inflammation which includes the production of free radicals. Multiple transcript variants encoding different isoforms have been characterized for this gene. [provided by RefSeq, Sep 2015]

Database

Research Area

DATA IMAGES

Anti-NRF2 antibody [HL3183] used in Western Blot (WB). GTX640822

GTX640822 WB Image

Orthogonal

Untreated (–) and treated (+) Neuro2A whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with NRF2 antibody [HL3183] (GTX640822) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-NRF2 antibody [HL3183] used in Western Blot (WB). GTX640822

GTX640822 WB Image

Orthogonal

Untreated (–) and treated (+) Rat2 whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with NRF2 antibody [HL3183] (GTX640822) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-NRF2 antibody [HL3183] used in Western Blot (WB). GTX640822

GTX640822 WB Image

Orthogonal

Untreated (–) and treated (+) HepG2 whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with NRF2 antibody [HL3183] (GTX640822) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

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REVIEW

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Package List Price ($)
$ 399
$ 169
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Custom Conjugations