APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
1:500~1:1000 |
1:50~1:100 |
Not tested in other applications.
Calculated MW
PROPERTIES
Form
Liquid
Buffer
PBS, 150mM NaCl, 50% Glycerol
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
Batch dependent (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
The antiserum was produced against synthesized peptide derived from human NT5C3 (11-60).
Purification
Purified by antigen-affinity chromatography
From serum
Conjugation
Unconjugated
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
5'nucleotidase, cytosolic IIIA , NT5C3 , NT5C3A , P5'N1 , P5N1 , PNI , POMP , PSN1 , UMPH , UMPH1 , cNIII , hUMP1 , p36 , 5'-nucleotidase, cytosolic IIIA
Cellular Localization
Cytoplasm,Endoplasmic reticulum
Background
This gene encodes a member of the 5'-nucleotidase family of enzymes that catalyze the dephosphorylation of nucleoside 5'-monophosphates. The encoded protein is the type 1 isozyme of pyrimidine 5' nucleotidase and catalyzes the dephosphorylation of pyrimidine 5' monophosphates. Mutations in this gene are a cause of hemolytic anemia due to uridine 5-prime monophosphate hydrolase deficiency. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene, and pseudogenes of this gene are located on the long arm of chromosomes 3 and 4. [provided by RefSeq, Mar 2012]
Database
Research Area
DATA IMAGES
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GTX87639 IHC-P Image
IHC-P analysis of human brain tissue using GTX87639 NT5C3 antibody. The picture on the right is blocked with the synthesized peptide.
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GTX87639 WB Image
WB analysis of Jurkat and COLO205 cell lysates using GTX87639 NT5C3 antibody. The lane on the right is blocked with the synthesized peptide.
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REFERENCE
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REVIEW
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