Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
Assay dependent |
Assay dependent |
1:20 |
Not tested in other applications.
Calculated MW
Product Note
This antibody reacts with PKC lambda immunoprecipitates, indicating cross-reactivity for PKC lambda [pT563]. PKC zeta [pT56] (83% homologous) has been shown to cross-react by peptide competition. Peptide competition also suggests that this antibody may partially cross-react with PKC beta 1 [pS642] (58% homologous) and PKC nu [pT655] (42% homologous).
Form
Liquid
Buffer
PBS, 0.1% BSA, 50% Glycerol
Preservative
0.05% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
Batch dependent (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Synthetic phosphopeptide derived from a region of human PKC iota that contains threonine 563.
Purification
Purified by antigen-affinity chromatography
Conjugation
Unconjugated
RRID
AB_384130
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
Synonyms
protein kinase C iota , DXS1179E , PKCI , nPKC-iota
Cellular Localization
Cytoplasm,Membrane,Endosome,Nucleus
Background
This gene encodes a member of the protein kinase C (PKC) family of serine/threonine protein kinases. The PKC family comprises at least eight members, which are differentially expressed and are involved in a wide variety of cellular processes. This protein kinase is calcium-independent and phospholipid-dependent. It is not activated by phorbolesters or diacylglycerol. This kinase can be recruited to vesicle tubular clusters (VTCs) by direct interaction with the small GTPase RAB2, where this kinase phosphorylates glyceraldehyde-3-phosphate dehydrogenase (GAPD/GAPDH) and plays a role in microtubule dynamics in the early secretory pathway. This kinase is found to be necessary for BCL-ABL-mediated resistance to drug-induced apoptosis and therefore protects leukemia cells against drug-induced apoptosis. There is a single exon pseudogene mapped on chromosome X. [provided by RefSeq, Jul 2008]
Database
Research Area