APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
1/500 - 1/2000 |
1/200 - 1/400 |
1/10000 |
Not tested in other applications.
Calculated MW
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
0.05% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Purified recombinant fragment of human RAP1A expressed in E. Coli.
Purification
Protein G Purified
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
RAP1A, member of RAS oncogene family , C21KG , G-22K , KREV-1 , KREV1 , RAP1 , SMGP21
Cellular Localization
Cell membrane,Cytoplasm,Cell junction
Background
The product of this gene belongs to the family of RAS-related proteins. These proteins share approximately 50% amino acid identity with the classical RAS proteins and have numerous structural features in common. The most striking difference between RAP proteins and RAS proteins resides in their 61st amino acid: glutamine in RAS is replaced by threonine in RAP proteins. The product of this gene counteracts the mitogenic function of RAS because it can interact with RAS GAPs and RAF in a competitive manner. Two transcript variants encoding the same protein have been identified for this gene. [provided by RefSeq, Jul 2008]
Database
Research Area
DATA IMAGES
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GTX60585 FACS Image
FACS analysis of A431 cells using GTX60585 RAP1A antibody [5F8]. Green : RAP1A Red : negative control
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GTX60585 WB Image
WB analysis of HEK293 (1) and RAP1A (AA: 28-180)-hIgGFc transfected HEK293 (2) cell lysate using GTX60585 RAP1A antibody [5F8].
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GTX60585 ELISA Image
ELISA analysis of antigen using GTX60585 RAP1A antibody [5F8].
Black : Control antigen 100ng
Purple : Antigen 10ng
Blue : Antigen 50ng
Red : Antigen 100ng
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GTX60585 WB Image
WB analysis of human RAP1A (AA: 28-180) recombinant protein using GTX60585 RAP1A antibody [5F8].
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REFERENCE
There are currently no references for RAP1A antibody [5F8] (GTX60585). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for RAP1A antibody [5F8] (GTX60585). Be the first to share your experience with this product.