RbAp48 + RbAp46 antibody [15G12]

Cat. No. GTX70236

Reference 9

Host	Mouse
Clonality	Monoclonal
Clone Name	15G12
Isotype	IgG2a
Application	WB, ICC/IF, IHC-P, IP, EMSA
Reactivity	Human, Mouse
Package	100 μl ($399)

See all RbAp48 + RbAp46 products

View product citations for antibody GTX70236 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500-1:3000
ICC/IF	1:100-1:1000
IHC-P	1:100-1:1000
IP	0.2 - 1 μg/ml
EMSA	Assay dependent

Not tested in other applications.

Calculated MW

46 and 48 kDa. ( Note )

Positive Control

K562 , THP-1 , HL-60

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

No Preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

The complete coding region (amino acids 1-425) of RbAp48 expressed in E. coli.

Purification

Protein G purified
From tissue culture supernatant

Conjugation

Unconjugated

RRID

AB_372873

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Cellular Localization

Nuclear

Background

RbAp48-15G12 recognizes full length RbAp48 (48 kDa) and RbAp46 (46 kDa), related nuclear proteins first isolated by their ability to interact with the carboxyl-terminus of the retinoblastoma protein (Rb). These two proteins are highly homologous at the amino acid level and are related functionally to members of the WD (Trp-Asp) repeat family of proteins. RbAp48 is one of the three subunits of CAF-1, can bind to histone H4 and is a subunit of human histone deacetylase HD1. Both RbAp48 and RbAp46 interact with HDAC1 and mSin3 in vivo.

Research Area

DATA IMAGES

GTX70236 ICC/IF Image

RbAp48 + RbAp46 antibody [15G12] detects RbAp48 + RbAp46 protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: RbAp48 + RbAp46 stained by RbAp48 + RbAp46 antibody [15G12] (GTX70236) diluted at 1:500.
Red: beta Tubulin, a cytoskeleton marker, stained by beta Tubulin antibody (GTX101279) diluted at 1:1000.
Scale bar= 10μm.

GTX70236 IHC-P Image

RbAp48 + RbAp46 antibody [15G12] detects RbAp48 + RbAp46 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded human cervical carcinoma.
RbAp48 + RbAp46 stained by RbAp48 + RbAp46 antibody [15G12] (GTX70236) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX70236 IHC-P Image

Immunohistochemical analysis of paraffin-embedded C2C12 xenograft, using RbAp48 + RbAp46(GTX70236) antibody at 1:200 dilution.

GTX70236 IHC-P Image

Immunohistochemical analysis of paraffin-embedded H1299 xenograft, using RbAp48 + RbAp46(GTX70236) antibody at 1:200 dilution.

GTX70236 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with RbAp48 + RbAp46 antibody [15G12] (GTX70236) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.