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SHC1 (phospho Tyr239/Tyr240) antibody

KO/KD Orthogonal
Anti-SHC1 (phospho Tyr239/Tyr240) antibody used in Western Blot (WB). GTX24890
Anti-SHC1 (phospho Tyr239/Tyr240) antibody used in IHC (Paraffin sections) (IHC-P). GTX24890
Anti-SHC1 (phospho Tyr239/Tyr240) antibody used in Western Blot (WB). GTX24890
Anti-SHC1 (phospho Tyr239/Tyr240) antibody used in Western Blot (WB). GTX24890
Anti-SHC1 (phospho Tyr239/Tyr240) antibody used in Western Blot (WB). GTX24890

Cat. No. GTX24890

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse, Rat
Package
50 μl ($399)
See all SHC1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500
IHC-P 1:10-1:50
Not tested in other applications.

Calculated MW

63 kDa. ( Note )

Product Note

This antibody cross-reacts with over-expressed insulin receptor protein in CHO cells.

PROPERTIES

Form

Liquid

Buffer

PBS, 0.1% BSA

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

The antiserum was produced against a chemically synthesized phosphopeptide derived from the region of human Shc that contains tyrosines 239 and 240.

Purification

Purified IgG

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

SHC adaptor protein 1 , SHC , SHCA

Cellular Localization

Cytoplasm

Background

This gene encodes three main isoforms that differ in activities and subcellular location. While all three are adapter proteins in signal transduction pathways, the longest (p66Shc) may be involved in regulating life span and the effects of reactive oxygen species. The other two isoforms, p52Shc and p46Shc, link activated receptor tyrosine kinases to the Ras pathway by recruitment of the GRB2/SOS complex. p66Shc is not involved in Ras activation. Unlike the other two isoforms, p46Shc is targeted to the mitochondrial matrix. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Feb 2011]

Database

Research Area

DATA IMAGES

Anti-SHC1 (phospho Tyr239/Tyr240) antibody used in Western Blot (WB). GTX24890

GTX24890 WB Image

Orthogonal

WB analysis of whole cell extracts (20 μg lysate) of A431 (Lane 1), treated for 10 minutes with 200 ng/ml of EGF (Lane 2), NIH/3T3 (Lane 3) and treated for 10 minutes with 200 ng/ml of EGF (Lane 4) using GTX24890 SHC1 (phospho Tyr239/Tyr240) antibody.
Dilution : 1:500

Anti-SHC1 (phospho Tyr239/Tyr240) antibody used in IHC (Paraffin sections) (IHC-P). GTX24890

GTX24890 IHC-P Image

IHC-P analysis of human colon carcinoma tissue using GTX24890 SHC1 (phospho Tyr239/Tyr240) antibody.
Right : Primary antibody
Left : Negative control without primary antibody
Antigen retrieval : 10mM sodium citrate (pH 6.0), microwaved for 8-15 min
Dilution : 1:20

Anti-SHC1 (phospho Tyr239/Tyr240) antibody used in Western Blot (WB). GTX24890

GTX24890 WB Image

Orthogonal

WB analysis of A431 stimulated with EGF (Lane 1-4) or left unstimulated (Lane 5) using GTX24890 SHC1 (phospho Tyr239/Tyr240) antibody.

Anti-SHC1 (phospho Tyr239/Tyr240) antibody used in Western Blot (WB). GTX24890

GTX24890 WB Image

Orthogonal

WB analysis of membrane enriched extracts (30 ug lysate) of A431 (Lane 1), A431 treated with EGF (200 ng/ml for 10 minutes) (Lane 2), A431 treated with Afatinib followed by EGF (0.5 uM for 6 hours, 200ng/ml for 10 minutes) (Lane 3), A549 (Lane 4), A549 treated with EGF (200 ng/ml for 10 minutes) (Lane 5) using GTX24890 SHC1 (phospho Tyr239/Tyr240) antibody.
Dilution : 1:500

Anti-SHC1 (phospho Tyr239/Tyr240) antibody used in Western Blot (WB). GTX24890

GTX24890 WB Image

KO/KD Orthogonal

WB analysis of 30 μg of A431 (Lane 1) treated with EGF (200 ng/ml for 10 minutes) (Lane 2), treated with Afatinib followed by EGF (0.5 uM of Afatinib for 6hrs, 200ng/ml for 10 minutes) (Lane 3), EGFR KO A431(Lane 4) treated with EGF (200 ng/ml for 10 minutes) (Lane 5), EGFR KO A431 treated with Afatinib followed by EGF (0.5 uM of Afatinib for 6hrs, 200ng/ml for 10 minutes) (Lane 6) whole cell extracts using GTX24890 SHC1 (phospho Tyr239/Tyr240) antibody.
Dilution : 1:500

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399