APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
1:500 - 1:2000 |
1:20 - 1:100 |
1:50 - 1:200 |
1:50 - 1:100 |
Not tested in other applications.
Calculated MW
PROPERTIES
Form
Liquid
Buffer
PBS, 50% Glycerol
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
Batch dependent (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
A phospho specific peptide corresponding to residues surrounding S957 of human SMC1A
Purification
Purified by affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
structural maintenance of chromosomes 1A , CDLS2 , DXS423E , SB1.8 , SMC1 , SMC1L1 , SMC1alpha , SMCB
Cellular Localization
Nucleus
Background
Proper cohesion of sister chromatids is a prerequisite for the correct segregation of chromosomes during cell division. The cohesin multiprotein complex is required for sister chromatid cohesion. This complex is composed partly of two structural maintenance of chromosomes (SMC) proteins, SMC3 and either SMC1B or the protein encoded by this gene. Most of the cohesin complexes dissociate from the chromosomes before mitosis, although those complexes at the kinetochore remain. Therefore, the encoded protein is thought to be an important part of functional kinetochores. In addition, this protein interacts with BRCA1 and is phosphorylated by ATM, indicating a potential role for this protein in DNA repair. This gene, which belongs to the SMC gene family, is located in an area of the X-chromosome that escapes X inactivation. Mutations in this gene result in Cornelia de Lange syndrome. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2013]
Database
Research Area
DATA IMAGES
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GTX55474 IHC-P Image
IHC-P analysis of human colon carcinoma tissue using GTX55474 SMC1 (phospho Ser957) antibody. Dilution : 1:100
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GTX55474 IP Image
IP analysis of HeLa cell lysate using GTX55474 SMC1 (phospho Ser957) antibody. Antibody amount : 3μg / 200μg lysate Dilution : 1:1000
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GTX55474 ICC/IF Image
ICC/IF analysis of U2OS cells using GTX55474 SMC1 (phospho Ser957) antibody. Blue : DAPI
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GTX55474 WB Image
WB analysis of HeLa and NIH/3T3 cells treated by UV for 15-30 minutes using GTX55474 SMC1 (phospho Ser957) antibody. Dilution : 1:1000 Loading : 25μg per lane
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GTX55474 ICC/IF Image
ICC/IF analysis of GFP-RNF168 transgenic U2OS cells using GTX55474 SMC1 (phospho Ser957) antibody. Green : GFP-RNF168 fusion protein expression for DNA damage marker Blue : DAPI
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GTX55474 WB Image
WB analysis of HeLa cells treated by UV at room temperature for 15-30 minutes using GTX55474 SMC1 (phospho Ser957) antibody. Dilution : 1:1000 Loading : 25μg per lane
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REFERENCE
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REVIEW
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