APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
0.05-0.2μg/ml |
3.75μg/ml |
Not tested in other applications.
Calculated MW
Positive Control
The peptide used to generate this antibody is available for purchase (GTX89846-PEP).
Product Note
This antibody is expected to recognise both isoform a (NP_071416.2; NP_690049.1) and isoform b (NP_690050.1).
Predict Reactivity
Mouse, Rat, Bovine, Dog, Pig(>80% identity)
PROPERTIES
Form
Liquid
Buffer
TBS, 0.5% BSA
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.50 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Peptide with sequence ATPYVPVPMPIGN-C, from the N Terminus of the protein sequence according to NP_071416.2; NP_690049.1; NP_690050.1.
Purification
Purified by ammonium sulphate precipitation followed by antigen affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
sorting nexin 16
Cellular Localization
Cytoplasm , Lysosome
Background
This gene encodes a member of the sorting nexin family. Members of this family contain a phox (PX) domain, which is a phosphoinositide binding domain, and are involved in intracellular trafficking. The protein encoded by this gene associates with late endosome membranes as is involved in tubule formation, cholesterol transport, and transport of tetraspanin CD81. The encoded protein also inhibits cell migration and tumorigenesis. [provided by RefSeq, Jan 2017]
Database
DATA IMAGES
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GTX89846 WB Image
WB analysis of Daudi lysate using GTX89846 SNX16 antibody, N-term. Dilution : 0.05μg/ml Loading : 35μg protein in RIPA buffer
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GTX89846 IHC-P Image
IHC-P analysis of human breast using GTX89846 SNX16 antibody, N-term. Antigen retrieval : citrate buffer pH 6 Dilution : 3.75μg/ml
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GTX89846 IHC-P Image
IHC-P analysis of human tonsil using GTX89846 SNX16 antibody, N-term. Antigen retrieval : citrate buffer pH 6 Dilution : 3.75μg/ml
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GTX89846 IHC-P Image
IHC-P analysis of human placenta using GTX89846 SNX16 antibody, N-term. Antigen retrieval : citrate buffer pH 6 Dilution : 3.75μg/ml
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GTX89846 WB Image
WB analysis of HEK293 overexpressing SNX16 (mock transfection in first lane) using GTX89846 SNX16 antibody, N-term.
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REFERENCE
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REVIEW
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