APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
1-2μg/ml |
1-2μg/ml for 30 minutes at RT |
1-2μg/10⁶ cells |
Note :
IHC-P
Staining of formalin-fixed tissues require heating tissue sections in 1mM EDTA, pH 7.5-8.5, for 45 min at 95ºC followed by cooling at RT for 20 minutes.
|
Not tested in other applications.
Calculated MW
Product Note
Recognizes a cluster of proteins between 70-80kDa, identified as tyrosinase. Occasionally a minor band at 55kDa is also detected. This MAb shows no cross-reaction with MAGE-1 and tyrosinase-related protein 1, TRP-1/gp75.
PROPERTIES
Form
Liquid
Buffer
PBS, 0.05% BSA
Preservative
0.05% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.2 mg/ml (Please refer to the vial label for the specific concentration.)
Immunogen
Recombinant tyrosinase protein
Purification
Protein A/G purified
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
ATN , CMM8 , OCA1 , OCA1A , OCAIA , SHEP3 , TYR , tyrosinase , Tyrosinase
Cellular Localization
Melanosome membrane,Melanosome
Background
The enzyme encoded by this gene catalyzes the first 2 steps, and at least 1 subsequent step, in the conversion of tyrosine to melanin. The enzyme has both tyrosine hydroxylase and dopa oxidase catalytic activities, and requires copper for function. Mutations in this gene result in oculocutaneous albinism, and nonpathologic polymorphisms result in skin pigmentation variation. The human genome contains a pseudogene similar to the 3' half of this gene. [provided by RefSeq, Oct 2008]
Database
Research Area
DATA IMAGES
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GTX35152 IHC-P Image
IHC-P analysis of human melanoma tissue using GTX35152 Tyrosinase antibody [SPM360].
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REFERENCE
There are currently no references for Tyrosinase antibody [SPM360] (GTX35152). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for Tyrosinase antibody [SPM360] (GTX35152). Be the first to share your experience with this product.