APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500-1:2000 |
| 1:100-1:500 |
| 1:50-1:200 |
Not tested in other applications.
Calculated MW
Predict Reactivity
Rat, Rabbit, Sheep, Bovine, Dog, Monkey(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS, 150mM NaCl, 50% Glycerol
Preservative
0.02% Sodium Azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
A synthesized peptide derived from human TYR(Accession P14679), corresponding to amino acid residues A481-L529.
Purification
From serum
Purity
Purified by antigen-affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
ATN,CMM8,OCA1,OCA1A,OCAIA,SHEP3,TYR,tyrosinase,Tyrosinase
Cellular Localization
Melanosome membrane,Melanosome
Background
The enzyme encoded by this gene catalyzes the first 2 steps, and at least 1 subsequent step, in the conversion of tyrosine to melanin. The enzyme has both tyrosine hydroxylase and dopa oxidase catalytic activities, and requires copper for function. Mutations in this gene result in oculocutaneous albinism, and nonpathologic polymorphisms result in skin pigmentation variation. The human genome contains a pseudogene similar to the 3' half of this gene. [provided by RefSeq, Oct 2008]
Database
Research Area
DATA IMAGES
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GTX04909 WB Image
WB analysis of various samples using GTX04909 Tyrosinase antibody.
Lane 1 : COLO205 whole cell lysate with blocking peptide treatment
Lane 2 : COLO205 whole cell lysate
Lane 3 : HepG2 whole cell lysate
Lane4 : B16F10 whole cell lysate
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GTX04909 ICC/IF Image
ICC/IF analysis of PFA-fixed HeLa cells using GTX04909 Tyrosinase antibody.
Red : Primary antibody
Green : beta tubulin
Blue : DAPI
Dilution : 1:200
Permeabilization : 0.1% Triton X-100
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GTX04909 IHC-P Image
IHC-P analysis of human kidney tissue using GTX04909 Tyrosinase antibody.
Antigen retrieval : Heat mediated citrate buffer
Dilution : 1:100
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REFERENCE
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REVIEW
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