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V5 tag antibody [GT1071] (HRP)

Anti-V5 tag antibody [GT1071] (HRP) used in Western Blot (WB). GTX628529-01

Cat. No. GTX628529-01

Reference 3

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 GT1071

Isotype

 IgG1

Application

 WB

Reactivity

 Species independent
Package
100 μl ($399),
25 μl ($169)
See all V5 tag products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:10000
Not tested in other applications.

Positive Control

V5-tagged protein expressing 293T cells

PROPERTIES

Form

Liquid

Buffer

0.05M Tris, 0.15M NaCl, 1%BSA

Preservative

0.005% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.81 mg/ml (Please refer to the vial label for the specific concentration.)

Immunogen

The immunogen used to generate this antibody corresponds to V5 tag

Purification

Affinity purified by Protein G.

Conjugation

Horseradish peroxidase(HRP)

RRID

AB_2888047

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

V5 tag antibody

Research Area

DATA IMAGES

Anti-V5 tag antibody [GT1071] (HRP) used in Western Blot (WB). GTX628529-01

GTX628529-01 WB Image

V5 tag antibody [GT1071] (HRP) detects V5 tag protein by Western blot analysis.
A. 30 μg 293T whole cell lysate/extract
B. 30 μg whole cell lysate/extract of V5 tagged protein expressing 293T cells
10 % SDS-PAGE
V5 tag antibody [GT1071] (HRP) (GTX628529-01) dilution: 1:5000

REFERENCE

Clone Reference

Diana Gumber et al. Elife 2020; 9 Selective activation of FZD7 promotes mesendodermal differentiation of human pluripotent stem cells.
Grainger S et al. Nat Cell Biol 2019; 21 (6):721-730 EGFR is required for Wnt9a-Fzd9b signalling specificity in haematopoietic stem cells.
Frose J et al. Cell Rep 2018; 24 (4):962-972 Epithelial-Mesenchymal Transition Induces Podocalyxin to Promote Extravasation via Ezrin Signaling.
Submit a Reference

REVIEW

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SDS
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169