APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
1:500-1:3000 |
Not tested in other applications.
Calculated MW
Positive Control
K562 , Neuro2A , C8D30 , NIH-3T3 , C2C12 , Rat2
Product Note
This antibody is raised against mouse c-Abl (P00520-1) phosphorylated at Tyr226. Based on sequence homology, it is predicted to react with human c-Abl when phosphorylated at the corresponding residues - c-Abl (isoform a) Tyr226 or c-Abl (isoform b) Tyr245.
Predict Reactivity
Bovine(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS, 1% BSA, 20% Glycerol
Preservative
0.025% ProClin 300
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1.08 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Mouse
Immunogen
Carrier-protein conjugated synthetic peptide surrounding phospho Tyr245 of mouse c-Abl. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_2887161
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
c-abl oncogene 1, non-receptor tyrosine kinase , AI325092 , Abl , E430008G22Rik , c-Abl
Cellular Localization
Cytoplasm , cytoskeleton , Nucleus
Background
Protein kinase that regulates key processes linked to cell growth and survival. Regulates cytoskeleton remodeling during cell differentiation, cell division and cell adhesion. Localizes to dynamic actin structures, and phosphorylates CRK and CRKL, DOK1, and other proteins controlling cytoskeleton dynamics. Regulates DNA repair potentially by activating the proapoptotic pathway when the DNA damage is too severe to be repaired. Phosphorylates PSMA7 that leads to an inhibition of proteasomal activity and cell cycle transition blocks.
Database
Research Area
DATA IMAGES
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GTX133980 WB Image
Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with c-Abl (phospho Tyr245) antibody (GTX133980) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX133980 WB Image
Untreated (–) and treated (+) K562 whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with c-Abl (phospho Tyr245) antibody (GTX133980) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX133980 WB Image
Whole cell extract (30 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with c-Abl (phospho Tyr245) antibody (GTX133980) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX133980 WB Image
Untreated (–) and treated (+) C2C12 whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with c-Abl (phospho Tyr245) antibody (GTX133980) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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REFERENCE
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REVIEW
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