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HKDC1 antibody

Anti-HKDC1 antibody used in Western Blot (WB). GTX111506
Anti-HKDC1 antibody used in IHC (Paraffin sections) (IHC-P). GTX111506
Anti-HKDC1 antibody used in IHC (Paraffin sections) (IHC-P). GTX111506
Anti-HKDC1 antibody used in Western Blot (WB). GTX111506
Anti-HKDC1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX111506

Cat. No. GTX111506

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P

Reactivity

Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

103 kDa. ( Note )

Positive Control

293T

Predict Reactivity

Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.45 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human HKDC1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2885309

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

hexokinase domain containing 1

Database

Research Area

DATA IMAGES

Anti-HKDC1 antibody used in Western Blot (WB). GTX111506

GTX111506 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with HKDC1 antibody (GTX111506) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Anti-HKDC1 antibody used in IHC (Paraffin sections) (IHC-P). GTX111506

GTX111506 IHC-P Image

HKDC1 antibody detects HKDC1 protein at cytoplasm in mouse duodenum by immunohistochemical analysis.
Sample: Paraffin-embedded mouse duodenum.
HKDC1 antibody (GTX111506) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-HKDC1 antibody used in IHC (Paraffin sections) (IHC-P). GTX111506

GTX111506 IHC-P Image

HKDC1 antibody detects HKDC1 protein at cytoplasm in rat brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
HKDC1 antibody (GTX111506) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-HKDC1 antibody used in Western Blot (WB). GTX111506

GTX111506 WB Image

Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with HKDC1 antibody (GTX111506) diluted at 1:2000.

Anti-HKDC1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX111506

GTX111506 ICC/IF Image

HKDC1 antibody detects HKDC1 protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: HKDC1 protein stained by HKDC1 antibody (GTX111506) diluted at 1:500.
Blue: Hoechst 33342 staining.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169