APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
1:500-1:10000 |
1:100-1:1000 |
1:100-1:1000 |
Assay dependent |
Not tested in other applications.
Calculated MW
Positive Control
HepG2 , mouse brain , rat brain
Predict Reactivity
Bovine, Pig, Xenopus laevis, Chimpanzee, Rhesus Monkey(>80% identity)
PROPERTIES
Form
Liquid
Buffer
0.1M Tris, 0.1M Glycine, 10% Glycerol
Preservative
0.01% Thimerosal
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the center region of human alpha SNAP. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_1950973
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
NSF attachment protein alpha , SNAPA
Cellular Localization
Membrane; Peripheral membrane protein
Background
The 'SNARE hypothesis' is a model explaining the process of docking and fusion of vesicles to their target membranes. According to this model, membrane proteins from the vesicle (v-SNAREs) and proteins from the target membrane (t-SNAREs) govern the specificity of vesicle targeting and docking through mutual recognition. Once the 2 classes of SNAREs bind to each other, they form a complex that recruits the general elements of the fusion apparatus, namely NSF (N-ethylmaleimide-sensitive factor) and SNAPs (soluble NSF-attachment proteins), to the site of membrane fusion, thereby forming the 20S fusion complex. Alpha- and gamma-SNAP are found in a wide range of tissues and act synergistically in intra-Golgi transport. The sequence of the predicted 295-amino acid human protein encoded by NAPA shares 37%, 60%, and 67% identity with the sequences of yeast, Drosophila, and squid alpha-SNAP, respectively. Platelets contain some of the same proteins, including NSF, p115/TAP, alpha-SNAP, gamma-SNAP, and the t-SNAREs syntaxin-2 and syntaxin-4, that are used in many vesicular transport processes in other cell types. Platelet exocytosis uses a molecular mechanism similar to that used by other secretory cells, such as neurons, although the proteins used by the platelet and their modes of regulation may be quite different. [provided by RefSeq]
Database
Research Area
DATA IMAGES
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GTX106626 WB Image
Sample(30 ug whole cell lysate) A:Hep G2 (GTX27900) 10% SDS PAGE GTX106626 diluted at 1:1000
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GTX106626 WB Image
Various tissue extracts (50 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with alpha SNAP antibody [N2C3] (GTX106626) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX106626 ICC/IF Image
alpha SNAP antibody [N2C3] detects alpha SNAP protein by immunofluorescent analysis. Sample: DIV10 rat E18 primary hippocampal neurons were fixed in 4% paraformaldehyde at RT for 15 min. Green: alpha SNAP protein stained by alpha SNAP antibody [N2C3] (GTX106626) diluted at 1:500. Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT1338] (GTX631831) diluted at 1:500. Blue: Fluoroshield with DAPI (GTX30920).
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GTX106626 IHC-P Image
alpha SNAP antibody [N2C3] detects alpha SNAP protein at cytosol on mouse lung by immunohistochemical analysis. Sample: Paraffin-embedded mouse lung. alpha SNAP antibody [N2C3] (GTX106626) dilution: 1:500.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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GTX106626 ICC/IF Image
Immunofluorescence analysis of methanol-fixed A431, using alpha SNAP(GTX106626) antibody at 1:200 dilution.
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GTX106626 ICC/IF Image
The data was published in the journal Evid Based Complement Alternat Med in 2013.PMID: 23573126
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REFERENCE
REVIEW
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