APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
1:500-1:3000 |
1:100-1:1000 |
1:100-1:1000 |
Not tested in other applications.
Calculated MW
Positive Control
HeLaS3 , Molt-4 , 293T , A431 , HeLa , HepG2
Predict Reactivity
Zebrafish, Bovine, Chicken, Xenopus tropicalis, Chimpanzee(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS, 20% Glycerol
Preservative
0.01% Thimerosal
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human CSN1. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_1240665
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
G protein pathway suppressor 1 , COPS1 , CSN1 , SGN1
Cellular Localization
Cytoplasm , Nucleus
Background
This gene is known to suppress G-protein and mitogen-activated signal transduction in mammalian cells. The encoded protein shares significant similarity with Arabidopsis FUS6, which is a regulator of light-mediated signal transduction in plant cells. Two alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]
Database
Research Area
DATA IMAGES
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GTX100291 IHC-P Image
CSN1 antibody [C3], C-term detects CSN1 protein at nucleus on mouse stomach by immunohistochemical analysis. Sample: Paraffin-embedded mouse stomach. CSN1 antibody [C3], C-term (GTX100291) dilution: 1:500.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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GTX100291 IHC-P Image
CSN1 antibody [C3], C-term detects CSN1 protein at nucleus on rat brain stem by immunohistochemical analysis. Sample: Paraffin-embedded rat brain stem. CSN1 antibody [C3], C-term (GTX100291) dilution: 1:500.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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GTX100291 IHC-P Image
Immunohistochemical analysis of paraffin-embedded A549 xenograft, using CSN1(GTX100291) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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GTX100291 WB Image
CSN1 antibody detects CSN1 protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with CSN1 antibody (GTX100291) diluted by 1:1000.
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GTX100291 WB Image
Sample(30 μg of whole cell lysate) A:HeLa S3 (GTX14654) B:MOLT4 (GTX27912) 15% SDS PAGE GTX100291 diluted at 1:1000
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GTX100291 IHC-P Image
CSN1 antibody [C3], C-term detects CSN1 protein at nucleus on mouse colon by immunohistochemical analysis. Sample: Paraffin-embedded mouse colon. CSN1 antibody [C3], C-term (GTX100291) dilution: 1:500.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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GTX100291 ICC/IF Image
Immunofluorescence analysis of paraformaldehyde-fixed A431, using CSN1(GTX100291) antibody at 1:500 dilution.
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REFERENCE
There are currently no references for CSN1 antibody [C3], C-term (GTX100291). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for CSN1 antibody [C3], C-term (GTX100291). Be the first to share your experience with this product.